HLA-DMA Polyclonal antibody, PBS Only

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  WB analysis of rat spleen using 11838-1-AP (same clone as 11838-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 11838-1-AP (same clone as 11838-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11838-1-AP (HLA-DMA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 11838-1-AP (same clone as 11838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11838-1-AP (HLA-DMA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11838-1-PBS in a different storage buffer formulation.

HLA-DMA Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11838-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

D6S222E, DMA, DMA protein, DMA protein MHC class II antigen Major histocompatibility complex, class II, DM alpha cDNA FLJ75740, highly similar to Homo sapiens major histocompatibility complex, class II, DM alpha, hCG_2001572

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Product Information

11838-1-PBS targets HLA-DMA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HLA-DMA fusion protein Ag2452
• Full Name: major histocompatibility complex, class II, DM alpha
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC026279
• Gene Symbol: HLA-DMA
• Gene ID (NCBI): 3108
• RRID: AB_874186
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28067
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides. This antibody recognizes the 33kd HLA-DMA and the 50-60kd dimer in Western blotting.