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  • KD/KO Validated

HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 14670-1-AP


CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Tested Applications

Positive WB detected inRaji cells, human placenta tissue, human spleen tissue, A431 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14670-1-AP targets HLA-F in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6335
Full Name major histocompatibility complex, class I, F
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC062991
Gene Symbol HLA-F
Gene ID (NCBI) 3134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)


Product Specific Protocols
WB protocol for HLA-F antibody 14670-1-APDownload protocol
IHC protocol for HLA-F antibody 14670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Kidney Int

Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells.

Authors - Tobias F Koyro

Int J Cancer

Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.

Authors - Zhang X X

J Immunol

Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.

Authors - Louis Marcellin

Int J Biol Sci

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

Authors - Xin Chen
  • KD Validated

Lung Cancer

HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.

Authors - Lin Aifen A

Oncol Lett

Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma.

Authors - Yongfu Xu