HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 55379-1-AP

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F



Tested Applications

Positive WB detected inRaji cells, A431 cells, human placenta tissue, Jurkat cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

55379-1-AP targets HLA-F in WB,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa, 66-70 kDa
GenBank accession numberNM_018950
Gene symbol HLA-F
Gene ID (NCBI) 3134
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
WB protocol for HLA-F antibody 55379-1-APDownload protocol
Standard Protocols
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