HMG20A Polyclonal antibody

• Featured Product
• KD/KO Validated

HMG20A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 12085-2-AP

Synonyms

HMGX1, HMG domain-containing protein HMGX1, HMG domain-containing protein 1, HMG box-containing protein 20A, HMG box containing protein 20A

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12085-2-AP

  WB result of HMG20A antibody (12085-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20A transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12085-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12085-2-AP (HMG20A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12085-2-AP

  IP result of anti-HMG20A (IP:12085-2-AP, 4ug; Detection:12085-2-AP 1:500) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 12085-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12085-2-AP (HMG20A antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12085-2-AP (HMG20A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of HepG2 using 12085-2-AP

  Immunofluorescent analysis of HepG2 cells, using HMG20A antibody 12085-2-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12085-2-AP targets HMG20A in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HMG20A fusion protein Ag2718
• Full Name: high-mobility group 20A
• Calculated Molecular Weight: 347 aa, 40 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC021959
• Gene Symbol: HMG20A
• Gene ID (NCBI): 10363
• RRID: AB_2117587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation

Publications

Species	Application	Title
human	WB,IP	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther ; KD Validated
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
mouse	WB	Cancer Cell Int; Circular RNA ABCB10 promotes hepatocellular carcinoma progression by increasing HMG20A expression by sponging miR-670-3p.; Authors - Yu Fu
human	IHC	Biochim Biophys Acta; Interactome of the inhibitory isoform of the nuclear transporter Importin 13.; Authors - Shadma Fatima
mouse	WB,IHC,IF,IP	Biochim Biophys Acta; The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins.; Authors - Davies Rebecca G RG