HMG20B Polyclonal antibody

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  WB analysis of HeLa using 14582-1-AP

  WB result of HMG20B antibody (14582-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HMG20B transfected HeLa cells.

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  WB analysis of HeLa using 14582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14582-1-AP (HMG20B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14582-1-AP

  IP result of anti-HMG20B (IP:14582-1-AP, 4ug; Detection:14582-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IHC staining of human colon cancer using 14582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14582-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14582-1-AP (HMG20B antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14582-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14582-1-AP (HMG20B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

HMG20B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14582-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

BRAF25, BRAF35, BRCA2 associated factor 35, BRCA2-associated factor 35, high mobility group 20B

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

14582-1-AP targets HMG20B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6103

  Product name: Recombinant human HMG20B protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 73-284 aa of BC002552

  Sequence: PVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYM

• Full Name: high-mobility group 20B
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002552
• Gene Symbol: HMG20B
• Gene ID (NCBI): 10362
• RRID: AB_2117601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P0W2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther
human	WB	Sci Adv; Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.; Authors - Roberto Ravasio
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
mouse	ChIP	Haematologica; The DNA binding factor Hmg20b is a repressor of erythroid differentiation.; Authors - Esteghamat Fatemehsadat F
human	WB,IP,IHC	Med; Inactivation of TACC2 epigenetically represses CDKN1A and confers sensitivity to CDK inhibitors; Authors - Zhi-Rui Lin
human	WB,IP	Mol Cancer; Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma; Authors - Jing Yang