Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14582-1-AP targets HMG20B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6103 Product name: Recombinant human HMG20B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 73-284 aa of BC002552 Sequence: PVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYM Predict reactive species |
| Full Name | high-mobility group 20B |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC002552 |
| Gene Symbol | HMG20B |
| Gene ID (NCBI) | 10362 |
| RRID | AB_2117601 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P0W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation [PMID:10449787]. The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse [PMID:15240883]. HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure [PMID:19997604].
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HMG20B antibody 14582-1-AP | Download protocol |
| IHC protocol for HMG20B antibody 14582-1-AP | Download protocol |
| IP protocol for HMG20B antibody 14582-1-AP | Download protocol |
| WB protocol for HMG20B antibody 14582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
HMG20B Polyclonal antibody (Cat# 14582-1-AP) has 6 citations published in Molecular Cancer, Nature Communications, Science Advances, Med, Haematologica, and EMBO Molecular Medicine. The cited research spans cancer biology (soft tissue sarcoma, acute myeloid leukemia, Merkel cell carcinoma), developmental biology (head and heart development), and erythroid differentiation.
| Species | Application | Title |
|---|---|---|
Mol Cancer Loss of TACC2 impairs chemokine CCL3 and CCL4 expression and reduces response to anti-PD-1 therapy in soft tissue sarcoma | ||
Nat Commun The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs | ||
Sci Adv Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation. | ||
Med Inactivation of TACC2 epigenetically represses CDKN1A and confers sensitivity to CDK inhibitors | ||
EMBO Mol Med LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma. |
