HMGB1 Monoclonal antibody

HMGB1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

23

CloneNo.

1D12A6

Cat no : 66525-1-Ig

Synonyms

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1

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Validation Data Gallery

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  WB analysis using 66525-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66525-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66525-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66525-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66525-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66525-1-Ig (HMGB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 12 publications below
• CoIP: See 1 publications below

Product Information

66525-1-Ig targets HMGB1 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: HMGB1 fusion protein Ag1264
• Full Name: high-mobility group box 1
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 30 kDa
• GenBank accession number: BC003378
• Gene symbol: HMGB1
• Gene ID (NCBI): 3146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Nat Microbiol; The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.; Authors - Tomas Doyle
human,mouse	WB,IF	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
mouse	IF, WB	Stem Cell Res Ther; Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation.; Authors - Wenqian Wei
human	WB	Front Immunol; Lysophosphatidylcholine Induces NLRP3 Inflammasome-Mediated Foam Cell Formation and Pyroptosis in Human Monocytes and Endothelial Cells.; Authors - Rafael Corrêa
human	WB,IF	Int J Biol Sci; A marine-derived small molecule induces immunogenic cell death against triple-negative breast cancer through ER stress-CHOP pathway.; Authors - Haiyan Wen