HMGB1 Monoclonal antibody

HMGB1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D12A6

Cat no : 66525-1-Ig

Synonyms

high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue, human breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66525-1-Ig targets HMGB1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HMGB1 fusion protein Ag1264
Full Name high-mobility group box 1
Calculated molecular weight 25 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003378
Gene symbol HMGB1
Gene ID (NCBI) 3146
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.

Protocols

Product Specific Protocols
WB protocol for HMGB1 antibody 66525-1-IgDownload protocol
IHC protocol for HMGB1 antibody 66525-1-IgDownload protocol
IF protocol for HMGB1 antibody 66525-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.

Authors - Haiyan Cui
humanIF

Neurotox Res

PMID: 31858421

Authors - Min Huang
mouseWB,IF

Int Immunopharmacol

Saikosaponin-d attenuated lipopolysaccharide-induced depressive-like behaviors via inhibiting microglia activation and neuroinflammation.

Authors - Jing Su
mouseIF

Int Immunopharmacol

SIRT1-regulated HMGB1 release is partially involved in TLR4 signal transduction: A possible anti-neuroinflammatory mechanism of resveratrol in neonatal hypoxic-ischemic brain injury.

Authors - Kai Le
mouseWB,IHC

Nat Microbiol

The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.

Authors - Tomas Doyle