HMGB1 Monoclonal antibody
HMGB1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Human, mouse and More (2)
WB, IHC, CoIP, ELISA
Cat no : 66525-1-Ig
|Positive WB detected in||HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
|WB||See 15 publications below|
|IHC||See 3 publications below|
|IF||See 12 publications below|
|CoIP||See 1 publications below|
66525-1-Ig targets HMGB1 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||HMGB1 fusion protein Ag1264|
|Full Name||high-mobility group box 1|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC003378|
|Gene ID (NCBI)||3146|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.
|Product Specific Protocols|
|WB protocol for HMGB1 antibody 66525-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry.
Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.
J Mater Chem B
Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating
Stem Cell Res Ther
Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation.
Lysophosphatidylcholine Induces NLRP3 Inflammasome-Mediated Foam Cell Formation and Pyroptosis in Human Monocytes and Endothelial Cells.
Int J Biol Sci
A marine-derived small molecule induces immunogenic cell death against triple-negative breast cancer through ER stress-CHOP pathway.