HMGB1 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66525-1-Ig |CloneNo.: 1D12A6

  • Print page
  • Download PDF

-_-

Freight/Packing

Con: 150 μg/150 μl

Choose size:

Please visit your regions distributor:


Species specificity:
Human

Positive WB detected in:
Jurkat cells, HeLa cells, HepG2 cells

Positive IHC detected in:
human liver cancer tissue, human tonsillitis tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:250-1:1000
IF : 1:50-1:500

Product Information


Source:
Mouse

Purification method:
Protein G purification

Isotype:
IgG1

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
high-mobility group box 1

Calculated molecular weight:
25 kDa

Observed molecular weight:
30 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
HMGB1

Synonyms
DKFZp686A04236, high mobility group box 1, High mobility group protein 1, High mobility group protein B1, HMG 1, HMG1, HMG3, HMGB1, SBP 1
Background

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize NUCLEOSOME formation and acts as a transcription-factor-like protein that regulates the expression of several genes[PMID: 18160415]. Once injury, infection or other inflammatory stimuli, activated macrophages, mature dendritic cells and natural killer cells can secret HMGB1, which act as a crucial cytokine[PMID: 20163887]. HMGB1 also involved in V(D)J recombination by acting as a cofactor of the RAG complex, stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS)[PMID: 19360789 ]. Act as a Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. HMGB1 (high mobility group box 1) modulates gene expression in the nucleus, but certain immune cells secrete HMGB1 as an extracellular Alarmin to signal tissue damage.The nuclear HMGB1 relocalizes to the extracellular milieu in senescent human and mouse cells in culture and in vivo, which stimulated cytokine secretion through TLR-4 signaling (23649808).This antibody is a rabbit polyclonal antibody raised against full length HMGB1 of human origin. The monomeric HMGB1 is 29 kDa and dimer is 58 kDa.


Back
to top