HNF1B Polyclonal antibody

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• HNF1B Polyclonal antibody (12533-1-AP)

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• KD/KO Validated

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12533-1-AP Copied!

Publications (39)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Synonyms

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

• 

  WB analysis of L02 using 12533-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MDCK using 12533-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 12533-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of L02 using 12533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: L02 cells, Jurkat cells, MDCK cells
• Positive IHC detected in: human renal cell carcinoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12533-1-AP targets HNF1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3232

  Product name: Recombinant human HNF1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-310 aa of BC017714

  Sequence: MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLETLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEEAAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPMKTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQSDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQRGVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFR

• Full Name: HNF1 homeobox B
• Calculated Molecular Weight: 557 aa, 61 kDa
• Observed Molecular Weight: 58-65 kDa
• GenBank Accession Number: BC017714
• Gene Symbol: HNF1B
• Gene ID (NCBI): 6928
• RRID: AB_2116758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35680
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

Publications

What published studies show

Anti-HNF1B antibody (Cat# 12533-1-AP) has accumulated 39 citations. These appear in journals such as Gastroenterology, Nature Communications, Molecular Cell, Oncogene, EMBO Reports, Cell Reports, Science Reports, eLife, Liver Transplantation, and others. Associated research fields include pancreatic cancer, hepatocellular carcinoma, ovarian clear cell carcinoma, prostate cancer, kidney disease, T cell biology, diabetes (MODY3/T2D), developmental biology, hepatic fibrosis, and SARS-CoV-2 infection.

Automatically generated from the 39 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Gastroenterology; Loss of Arid1a and Pten in Pancreatic Ductal Cells Induces Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway; Authors - Yuichi Fukunaga
		Nat Commun; Pancreatic β-cell regeneration in situ by the ALK3 agonist THR-123; Authors - Silvia Álvarez-Cubela
human	WB	Mol Cell; Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion; Authors - Elena Battistello
mouse	IHC	J Exp Clin Cancer Res; The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease.; Authors - Antonio Cigliano
mouse	WB,IHC	Cell Death Dis; Dimethyl fumarate promotes the degradation of HNF1B and suppresses the progression of clear cell renal cell carcinoma; Authors - Yue Dai ; KD Validated
human	IF	Biomater Res; Engineered Endometrial Clear Cell Cancer-on-a-Chip Reveals Early Invasion-Metastasis Cascade of Cancer Cells; Authors - Chengpan Li