HNF1B Polyclonal antibody

• Featured Product
• KD/KO Validated

HNF1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 12533-1-AP

Synonyms

TCF-2, TCF2, HNF-1-beta, HNF-1B, HNF 1 beta

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Validation Data Gallery

• 

  WB analysis of L02 using 12533-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDCK using 12533-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12533-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12533-1-AP (HNF1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12533-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12533-1-AP (HNF1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 12533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 12533-1-AP (HNF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, Jurkat cells, MDCK cells
• Positive IHC detected in: human renal cell carcinoma tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 11 publications below
• IF: See 3 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

12533-1-AP targets HNF1B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HNF1B fusion protein Ag3232
• Full Name: HNF1 homeobox B
• Calculated Molecular Weight: 557 aa, 61 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: BC017714
• Gene Symbol: HNF1B
• Gene ID (NCBI): 6928
• RRID: AB_2116758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hepatocyte nuclear factor-1-beta(HNF1B), or transcription factor-2(TCF2), is a member of the homeodomain-containing superfamily of transcription factors. Early expression of HNF1B(12533-1-AP) is seen in the kidney, liver, pancreas, thymus, lung, gut and genital tract. HNF1B can be a homodimer or a heterodimer with HNF1A. It's a transcription factor, probably by binding to the inverted palindrome 5'-GTTAATNATTAAC-3', and as a bookmarking factor that marks target genes for rapid transcriptional reactivation after mitosis. This is a rabbit polyclonal antibody raised against part chain of N-terminus of human HNF1B. HNF1B exists some isoforms with MV 61 kDa, 58 kDa, 46 kDa and 44 kDa.

Publications

Species	Application	Title
human	IHC	Elife; Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.; Authors - Marko Z Nikolić
human	WB,IHC	Oncogene; HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.; Authors - Jianqing Wang ; KD Validated
mouse	IF	Development; Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.; Authors - Asha Seth
pig	IF	Sci Rep; Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.; Authors - Phillip L Lewis
human	WB,IHC	J Cell Mol Med; HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.; Authors - Wei Lu
mouse	IHC	Sci Rep; ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.; Authors - Yiannis Drosos

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