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HNRNPK Polyclonal antibody
HNRNPK Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 11426-1-AP
|Positive WB detected in||MCF-7 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11426-1-AP targets HNRNPK in WB, RIP, IP, IHC, IF, FC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||deer, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPK fusion protein Ag1976|
|Full Name||heterogeneous nuclear ribonucleoprotein K|
|Calculated molecular weight||463 aa, 51 kDa|
|Observed molecular weight||55-65 kDa|
|GenBank accession number||BC014980|
|Gene ID (NCBI)||3190|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability
|Product Specific Protocols|
|WB protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IHC protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IF protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|IP protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|FC protocol for HNRNPK antibody 11426-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.
Cell Biol Int
Identification and analysis of glycogen synthase kinase 3 beta1 interactome.
J Mol Biol
Proteomic characterization of the nucleolar linker histone H1 interaction network.
hnRNP K is a novel ITAF that negatively regulates foot-and-mouth disease virus translation and replication and is antagonized by viral 3C protease.
J Proteome Res
Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
DEBJIT (Verified Customer) (09-17-2020)
WB: 1:2000IP: 2ug/mg cell lysate