HNRNPU Polyclonal antibody

HNRNPU Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16365-1-AP

Synonyms

hnRNP U, HNRNPU, HNRPU, p120, pp120, SAF A, SAFA, Scaffold attachment factor A, U21.1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse testis tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16365-1-AP targets HNRNPU in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPU fusion protein Ag9151
Full Name heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
Calculated molecular weight 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC003367
Gene symbol HNRNPU
Gene ID (NCBI) 3192
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

Protocols

Product Specific Protocols
WB protocol for HNRNPU antibody 16365-1-APDownload protocol
IHC protocol for HNRNPU antibody 16365-1-APDownload protocol
IF protocol for HNRNPU antibody 16365-1-APDownload protocol
IP protocol for HNRNPU antibody 16365-1-APDownload protocol
FC protocol for HNRNPU antibody 16365-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Prolif

Protein phosphatase 4 plays dual roles during cell proliferation.

Authors - Xiuqing Huang
humanIF

Cancer Res

DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing.

Authors - Songge Xing
humanWB

Dev Cell

Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.

Authors - Yanni Ma