Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse testis tissue|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16365-1-AP targets HNRNPU in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HNRNPU fusion protein Ag9151|
|Full Name||heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)|
|Calculated molecular weight||91 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC003367|
|Gene ID (NCBI)||3192|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).
|Product Specific Protocols|
|WB protocol for HNRNPU antibody 16365-1-AP||Download protocol|
|IHC protocol for HNRNPU antibody 16365-1-AP||Download protocol|
|IF protocol for HNRNPU antibody 16365-1-AP||Download protocol|
|IP protocol for HNRNPU antibody 16365-1-AP||Download protocol|
|FC protocol for HNRNPU antibody 16365-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
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Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.