HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

109

Cat no : 27282-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HO-1/HMOX1, HSP32

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Validation Data Gallery

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  WB analysis of HeLa using 27282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27282-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27282-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 27282-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27282-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27282-1-AP (HO-1/HMOX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 27282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27282-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27282-1-AP (HO-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, mouse spleen tissue, HepG2 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 109 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

The immunogen of 27282-1-AP is HO-1/HMOX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken, hamster, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HO-1/HMOX1 fusion protein Ag26172
• Full Name: heme oxygenase (decycling) 1
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Publications

Species	Application	Title
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang
mouse	WB	Free Radic Biol Med; Nrf2 and NF-κB/NLRP3 inflammasome pathways are involved in Prototheca bovis infections of mouse mammary gland tissue and mammary epithelial cells.; Authors - Wenpeng Zhao
mouse	WB	Free Radic Biol Med; Immune-responsive gene 1 protects against liver injury caused by concanavalin A via the activation Nrf2/HO-1 pathway and inhibition of ROS activation pathways.; Authors - Wenchang Yang
rat	WB	Free Radic Biol Med; Hinokitiol functions as a ferroptosis inhibitor to confer neuroprotection; Authors - Junmin Xi
mouse	WB	Free Radic Biol Med; Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion.; Authors - Ling Zeng
mouse	WB	Front Immunol; A Novel Derivative of the Natural Product Danshensu Suppresses Inflammatory Responses to Alleviate Caerulein-Induced Acute Pancreatitis.; Authors - Zhengnan Ren