HO-1/HMOX1 Polyclonal antibody

HO-1/HMOX1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 27282-1-AP

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HO-1, HSP32



Tested Applications

Positive WB detected inHeLa cells, A549 cells, mouse spleen tissue, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27282-1-AP is HO-1/HMOX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity hamster, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HO-1/HMOX1 fusion protein Ag26172
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC001491
Gene symbol HMOX1
Gene ID (NCBI) 3162
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).

Protocols

Product Specific Protocols
WB protocol for HO-1/HMOX1 antibody 27282-1-APDownload protocol
IHC protocol for HO-1/HMOX1 antibody 27282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
hamsterWB

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
mouseWB

Int Immunopharmacol

Oxyresveratrol prevents lipopolysaccharide/d-galactosamine-induced acute liver injury in mice.

Authors - Ya-Nan Jia
ratWB,IF

Oxid Med Cell Longev

Sodium Tanshinone IIA Sulfonate Attenuates Erectile Dysfunction in Rats with Hyperlipidemia.

Authors - Liren Zhong
mouseWB

Int J Nanomedicine

La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.

Authors - Lu Yuan
ratWB

World J Gastroenterol

Morin enhances hepatic Nrf2 expression in a liver fibrosis rat model.

Authors - Liang Sang
human,ratWB

Free Radic Biol Med

Neuroprotective effect of phosphocreatine on oxidative stress and mitochondrial dysfunction induced apoptosis in vitro and in vivo: Involvement of dual PI3K/Akt and Nrf2/HO-1 pathways.

Authors - Hailong Li