HO-1 Recombinant antibody

• Featured Product
• KD/KO Validated

HO-1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2M6

Cat no : 81281-1-RR

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32

---

Validation Data Gallery

• 

  WB analysis of HeLa using 81281-1-RR

  WB result of HO-1 antibody (81281-1-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HO-1 transfected HeLa cells.

• 

  WB analysis using 81281-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81281-1-RR (HO-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

Sample-dependent, check data in validation data gallery

Product Information

81281-1-RR targets HO-1 in WB, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: HO-1 fusion protein Ag1190
• Full Name: heme oxygenase (decycling) 1
• Calculated molecular weight: 33 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC001491
• Gene symbol: HMOX1
• Gene ID (NCBI): 3162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).