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HO-1 Recombinant antibody

HO-1 Recombinant Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat and More (3)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2M6

Cat no : 81281-1-RR

Synonyms

bK286B10, heme oxygenase (decycling) 1, Heme oxygenase 1, HMOX1, HO, HO 1, HO1, HSP32



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 13 publications below

Product Information

81281-1-RR targets HO-1 in WB, IP, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HO-1 fusion protein Ag1190
Full Name heme oxygenase (decycling) 1
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC001491
Gene symbol HMOX1
Gene ID (NCBI) 3162
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).

Protocols

Product Specific Protocols
WB protocol for HO-1 antibody 81281-1-RRDownload protocol
IP protocol for HO-1 antibody 81281-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Negative regulation of pro-apoptotic AMPK/JNK pathway by itaconate in mice with fulminant liver injury

Authors - Kerui Fan
mouseWB

Ecotoxicol Environ Saf

Yttrium chloride induces ferroptosis in cardiomyocytes via iron accumulation and triggers cardiac lipid peroxidation and inflammation that cause heart adverse events in mice

Authors - Liang Xiong
mouseWB

J Agric Food Chem

Lactoferrin Relieves Deoxynivalenol-Induced Oxidative Stress and Inflammatory Response by Modulating the Nrf2/MAPK Pathways in the Liver

Authors - Ping Hu
mouseWB

Int J Mol Sci

Red Ginseng Improves D-galactose-Induced Premature Ovarian Failure in Mice Based on Network Pharmacology

Authors - Zijing Shang
ratWB

Eur J Pharmacol

The protective effects of uric acid against myocardial ischemia via the Nrf2 pathway

Authors - Pengqiang Ma
mouseWB

Eur J Pharmacol

Kinsenoside alleviates oxidative stress-induced blood-brain barrier dysfunction via promoting Nrf2/HO-1 pathway in ischemic stroke

Authors - Nan Qiao