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HO-1 Recombinant antibody
HO-1 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Rat
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2M6
Cat no : 81281-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Sample-dependent, check data in validation data gallery |
Product Information
81281-1-RR targets HO-1 in WB, ELISA applications and shows reactivity with Human, Rat samples.
Tested Reactivity | Human, Rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | HO-1 fusion protein Ag1190 |
Full Name | heme oxygenase (decycling) 1 |
Calculated molecular weight | 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC001491 |
Gene symbol | HMOX1 |
Gene ID (NCBI) | 3162 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).
Protocols
Product Specific Protocols | |
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WB protocol for HO-1 antibody 81281-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |