|Positive WB detected in
|A549 cells, HeLa cells, HepG2 cells, U2OS cells, HSC-T6 cells, rat spleen tissue
|Positive IP detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
81281-1-RR targets HO-1 in WB, IP, ELISA applications and shows reactivity with Human, Rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|HO-1 fusion protein Ag1190
|heme oxygenase (decycling) 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663).
Cell Death Dis
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