HOOK1 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10871-1-AP (same clone as 10871-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10871-1-AP (same clone as 10871-1-PBS)

  IP result of anti-HOOK1 (IP:10871-1-AP, 4ug; Detection:10871-1-AP 1:500) with mouse brain tissue lysate 4680ug. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 10871-1-AP (same clone as 10871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10871-1-AP (HOOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 10871-1-AP (HOOK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HOOK1 antibody (10871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

HOOK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 10871-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Protein Hook homolog 1, hook homolog 1 (Drosophila), HK1, h-hook1, hHK1

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Product Information

10871-1-PBS targets HOOK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1311

  Product name: Recombinant human HOOK1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 377-728 aa of BC011621

  Sequence: QVQDLHVKLSSESKRADTLAFEMKRLEEKHEALLKEKERLIEQRDTLKETNEELRCSQVQQDHLNQTDASATKSYENLAAEIMPVEYREVFIRLQHENKMLRLQQEGSENERIEELQEQLEQKHRKMNELETEQRLSKERIRELQQQIEDLQKSLQEQGSKSEGESSSKLKQKLEAHMEKLTEVHEELQKKQELIEDLQPDINQNVQKINELEAALQKKDEDMKAMEERYKMYLEKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIEILESECKVAKFRDYEEKLIVSAWYNKSLAFQKLGMESRLVSGGGACSDTGACTPARSFLAQQRHITNTRRNLSVKVPATTSD

• Full Name: hook homolog 1 (Drosophila)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC011621
• Gene Symbol: HOOK1
• Gene ID (NCBI): 51361
• RRID: AB_2120812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJC3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.