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  • KD/KO Validated

HOPX Polyclonal antibody

HOPX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11419-1-AP

Synonyms

CAMEO, HOD, Homeodomain only protein, HOP, HOP homeobox, HOPX, LAGY, NECC1, OB1, Odd homeobox protein 1, SMAP31, TOTO



Tested Applications

Positive WB detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Product Information

11419-1-AP targets HOPX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOPX fusion protein Ag1979
Full Name HOP homeobox
Calculated molecular weight 73 aa, 8 kDa
Observed molecular weight 8-12 kDa
GenBank accession numberBC014225
Gene symbol HOPX
Gene ID (NCBI) 84525
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOPX (Homeodomain-only protein) gene has various synonyms including HOD, HOP, OB1, LAGY and NECC1. The protein encoded by this gene is an unusual homeodomain protein that lacks certain conserved residues required for DNA binding. HOPX has diverse effects on cardiac growth. Manipulation of Hopx function in murine models is associated with cardiac hypertrophy, dilation and fibrosis. HOPX protein acts as an antagonist to the serum response factor (SRF), which regulates the opposing processes of cell proliferation and differentiation. Overexpression of HOPX causes cardiac hypertrophy. HOPX protein can inhibit SRF-dependent transcriptional activation by recruiting histone deacetylase (HDAC) activity.

Protocols

Product Specific Protocols
WB protocol for HOPX antibody 11419-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

Cell stress in cortical organoids impairs molecular subtype specification.

Authors - Aparna Bhaduri
humanIF

Cell

Origins and Proliferative States of Human Oligodendrocyte Precursor Cells.

Authors - Wei Huang
humanIHC

Cancer Cell

Epigenomic State Transitions Characterize Tumor Progression in Mouse Lung Adenocarcinoma.

Authors - Lindsay M LaFave
human

Cancer Cell

Control of alveolar differentiation by the lineage transcription factors GATA6 and HOPX inhibits lung adenocarcinoma metastasis.

Authors - William K C Cheung
human,mouseIF

Nat Neurosci

Single-cell atlas of early human brain development highlights heterogeneity of human neuroepithelial cells and early radial glia.

Authors - Ugomma C Eze
human,mouseIHC,IF,WB

Nat Commun

YAP1/TAZ drives ependymoma-like tumour formation in mice.

Authors - Noreen Eder

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana (Verified Customer) (11-10-2020)

We recently published with this paper: PubMed ID: 32819430 Images are within the manuscript.Protocol information: We used Citirc Acid Buffer Antigen Retrieval

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Human Brain (fetal), Paraffin Processed
HOPX Antibody Immunohistochemistry validation (1:250 dilution) in Human Brain (fetal), Paraffin Processed (Cat no:11419-1-AP)
FH

Nicole (Verified Customer) (07-23-2019)

Used to mark stem cell cultures for differentiation into neural like cells

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Cell Tissue Type: Stem Cell culture