HSD17B4 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSD17B4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 15116-1-AP

Synonyms

D-bifunctional protein, D bifunctional protein, 17-beta-hydroxysteroid dehydrogenase 4, 17-beta-HSD 4, 17 beta HSD 4

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Validation Data Gallery

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  WB analysis using 15116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15116-1-AP

  WB result of HSD17B4 antibody (15116-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSD17B4 transfected HepG2 cells.

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  WB analysis of mouse brain using 15116-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15116-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15116-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15116-1-AP

  IP result of anti-HSD17B4 (IP:15116-1-AP, 4ug; Detection:15116-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15116-1-AP (HSD17B4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15116-1-AP (HSD17B4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 15116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15116-1-AP (HSD17B4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15116-1-AP

  Immunofluorescent analysis of Hela cells, using HSD17B4 antibody 15116-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse brain tissue, mouse heart tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue, mouse liver tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below

Product Information

15116-1-AP targets HSD17B4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSD17B4 fusion protein Ag7165
• Full Name: hydroxysteroid (17-beta) dehydrogenase 4
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 80 kDa, 45 kDa
• GenBank Accession Number: BC003098
• Gene Symbol: HSD17B4
• Gene ID (NCBI): 3295
• RRID: AB_2119959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSD17B4 (17-beta-hydroxysteroid dehydrogenase 4) is also named as Peroxisomal multifunctional enzyme type 2, D-bifuntional protein or multifunctional protein 2. It codes for a 80 kDa enzyme containing three distinct functional domains and is localized in peroxisomes. It is a bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids and catalyzing the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-brancked-chian fatty acids. After peroxisomal import, the full-length protein is proteolytically cleaved to yield a 35-kDa dehydrogenase subunit and a 45-kDa hydratase subunit containing the hydratase and SCP domains (PMID: 28868548, 24602372).

Publications

Species	Application	Title
mouse	WB	Commun Biol; Transmembrane protein 135 regulates lipid homeostasis through its role in peroxisomal DHA metabolism; Authors - Michael Landowski
mouse	WB	Neurobiol Dis; Peroxisomal multifunctional protein-2 deficiency causes neuroinflammation and degeneration of Purkinje cells independent of very long chain fatty acid accumulation.; Authors - Verheijden Simon S
	WB	Sci Rep; Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells.; Authors - Mitsuki Nozaki
mouse	WB	Cells; Cell Type-Selective Loss of Peroxisomal β-Oxidation Impairs Bipolar Cell but Not Photoreceptor Survival in the Retina.; Authors - Daniëlle Swinkels
human	WB	Mol Cells; The Peroxisomal Localization of Hsd17b4 Is Regulated by Its Interaction with Phosphatidylserine.; Authors - Sang-Ah Lee
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali