HSFY1 Polyclonal antibody
HSFY1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 11970-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human testis tissue |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | HSFY1 fusion protein Ag2592 |
Full Name | heat shock transcription factor, Y-linked 1 |
Calculated Molecular Weight | 401 aa, 45 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC055414 |
Gene Symbol | HSFY1 |
Gene ID (NCBI) | 86614 |
RRID | AB_2877810 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.
Protocols
Product Specific Protocols | |
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WB protocol for HSFY1 antibody 11970-1-AP | Download protocol |
IHC protocol for HSFY1 antibody 11970-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |