HSFY1 Polyclonal antibody

HSFY1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11970-1-AP

Synonyms

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2

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Validation Data Gallery

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  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11970-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11970-1-AP (HSFY1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 11970-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11970-1-AP (HSFY1 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSFY1 fusion protein Ag2592
• Full Name: heat shock transcription factor, Y-linked 1
• Calculated Molecular Weight: 401 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC055414
• Gene Symbol: HSFY1
• Gene ID (NCBI): 86614
• RRID: AB_2877810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.