HSFY1 Polyclonal antibody

HSFY1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11970-1-AP

Synonyms

HSF2 like, HSF2L, HSFY, HSFY1, HSFY2



Tested Applications

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11970-1-AP targets HSFY1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSFY1 fusion protein Ag2592
Full Name heat shock transcription factor, Y-linked 1
Calculated Molecular Weight 401 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC055414
Gene Symbol HSFY1
Gene ID (NCBI) 86614
RRIDAB_2877810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The HSFY gene is located in the male-specific region of chromosome Y (MSY), which is not involved in X-Y crossover events. HSFY gene is expressed exclusively in testis. HSFY is a DNA-binding protein that specifically binds heat shock promoter elements(HSE), and suggested to be implicated in spermatogenesis both in animals and humans.

Protocols

Product Specific Protocols
WB protocol for HSFY1 antibody 11970-1-APDownload protocol
IHC protocol for HSFY1 antibody 11970-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols