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HSP90AB1 Polyclonal antibody
HSP90AB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11405-1-AP
Validation Data Gallery
|Positive WB detected in||PC-3 cells, mouse brain tissue, Jurkat cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human pancreas cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11405-1-AP targets HSP90AB1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, deer, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP90AB1 fusion protein Ag1947|
|Full Name||heat shock protein 90kDa alpha (cytosolic), class B member 1|
|Calculated molecular weight||723 aa, 83 kDa|
|Observed molecular weight||83-90 kDa|
|GenBank accession number||BC014485|
|Gene ID (NCBI)||3326|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
|Product Specific Protocols|
|WB protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IHC protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IF protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IP protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Maria (Verified Customer) (07-06-2022)
Very good antibody, we achieved good results.
Kyosuke (Verified Customer) (06-12-2019)
it works good for WB.