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HSP90AB1 Polyclonal antibody

HSP90AB1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11405-1-AP

Synonyms

Heat shock protein HSP 90 beta, HS90B, HSP84, HSP90 BETA, HSP90AB1, HSP90B



Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue, Jurkat cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11405-1-AP targets HSP90AB1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, deer, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP90AB1 fusion protein Ag1947
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
Observed molecular weight 83-90 kDa
GenBank accession numberBC014485
Gene symbol HSP90AB1
Gene ID (NCBI) 3326
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Protocols

Product Specific Protocols
WB protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IHC protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IF protocol for HSP90AB1 antibody 11405-1-APDownload protocol
IP protocol for HSP90AB1 antibody 11405-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Res

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Authors - Shaojun Zhang
pigWB

Autophagy

Induction of autophagy and suppression of type I IFN secretion by CSFV.

Authors - Baoming Xie
humanWB

Haematologica

Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.

Authors - Stephan Michalik
humanWB

Anal Chem

Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.

Authors - Xiaolei Zhang
sheepIF

PLoS Pathog

A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.

Authors - Thomas Labadie
mouseWB

Pharmacol Res

GADD45β stabilized by direct interaction with HSP72 ameliorates insulin resistance and lipid accumulation.

Authors - Yunxia Dong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (07-06-2022)

Very good antibody, we achieved good results.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Fibroblasts - human
FH

Kyosuke (Verified Customer) (06-12-2019)

it works good for WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HEK293T