Tested Applications
| Positive WB detected in | PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11405-1-AP targets HSP90 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, hamster, sheep, deer |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1947 Product name: Recombinant human HSP90AB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 330-710 aa of BC014485 Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP Predict reactive species |
| Full Name | heat shock protein 90kDa alpha (cytosolic), class B member 1 |
| Calculated Molecular Weight | 723 aa, 83 kDa |
| Observed Molecular Weight | 83-90 kDa |
| GenBank Accession Number | BC014485 |
| Gene Symbol | HSP90AB1 |
| Gene ID (NCBI) | 3326 |
| RRID | AB_2121207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08238 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HSP90 antibody 11405-1-AP | Download protocol |
| IF protocol for HSP90 antibody 11405-1-AP | Download protocol |
| IHC protocol for HSP90 antibody 11405-1-AP | Download protocol |
| IP protocol for HSP90 antibody 11405-1-AP | Download protocol |
| WB protocol for HSP90 antibody 11405-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HSP90 (HSP90AB1) polyclonal antibody (Cat# 11405-1-AP) has 54 citations spanning journals including Nature Communications, Cell Metabolism, Cell Research, Neuron, Cancer Research, Journal of Clinical Investigation, Developmental Cell, PLoS Pathogens, and Journal of Virology. Research fields covered include oncology (lung, breast, prostate, bladder, colorectal, ovarian cancers), virology (SARS-CoV-2, bluetongue virus, porcine deltacoronavirus), neuroscience, immunology, metabolism, proteomics, and toxicology.
| Species | Application | Title |
|---|---|---|
Cell Metab Nondigestible stachyose binds membranous HSP90β on small intestinal epithelium to regulate the exosomal miRNAs: A new function and mechanism | ||
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. | ||
Cancer Res N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma | ||
Nat Commun Synthetic carbon-based lanthanide upconversion nanoparticles for enhanced photothermal therapy | ||
ACS Nano Near-Infrared Phototheranostic Iron Pyrite Nanocrystals Simultaneously Induce Dual Cell Death Pathways via Enhanced Fenton Reactions in Triple-Negative Breast Cancer |
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported positive results. One user (5/5) tested it at 1:2000 on human fibroblasts and described it as a "very good antibody" with good results. The other (3/5) used it at 1:1000 on HEK293T cells and confirmed it works well for WB. Overall, users find it reliable for Western Blot applications across different cell types and dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Maria (Verified Customer) (07-06-2022) | Very good antibody, we achieved good results.
|
Kyosuke (Verified Customer) (06-12-2019) | it works good for WB.
|
