Validation Data Gallery
|Positive WB detected in||PC-3 cells, mouse brain tissue, Jurkat cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human pancreas cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11405-1-AP targets HSP90AB1 in WB, IP, IHC, IF, FC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||deer, human, mouse, pig, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP90AB1 fusion protein Ag1947|
|Full Name||heat shock protein 90kDa alpha (cytosolic), class B member 1|
|Calculated molecular weight||723 aa, 83 kDa|
|Observed molecular weight||83-90 kDa|
|GenBank accession number||BC014485|
|Gene ID (NCBI)||3326|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.
|Product Specific Protocols|
|WB protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IHC protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IF protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|IP protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|FC protocol for HSP90AB1 antibody 11405-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.
Quantitative proteomic analysis reveals unfolded protein response involved in severe fever with thrombocytopenia syndrome virus infection.
J Enzyme Inhib Med Chem
Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.
Hepatic DNAJB9 Drives Anabolic Biasing to Reduce Steatosis and Obesity.
Eur J Pharmacol
Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes.
Int J Mol Med
Osteoking improves OP rat by enhancing HSP90‑β expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kyosuke (Verified Customer) (06-12-2019)
it works good for WB.