HSP90 Polyclonal antibody

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11405-1-AP

  WB result of HSP90AB1 antibody (11405-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

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  WB analysis using 11405-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11405-1-AP (HSP90AB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 11405-1-AP

  IP result of anti-HSP90AB1 (IP:11405-1-AP, 4ug; Detection:11405-1-AP 1:700) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human pancreas cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human breast cancer using 11405-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11405-1-AP (HSP90AB1 antibody) at dilution of 1:500 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 11405-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSP90AB1 antibody (11405-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 11405-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug HSP90AB1 Polyclonal antibody (11405-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

HSP90 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11405-1-AP

Publications(37)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Synonyms

HSP90AB1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HSP90B

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Tested Applications

• Positive WB detected in: PC-3 cells, HEK-293 cells, Jurkat cells, mouse brain tissue, MCF-7 cells, HeLa cells, mouse thymus tissue, rat thymus tissue, mouse heart tissue, rat brain tissue, rat heart tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 34 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Product Information

11405-1-AP targets HSP90 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, sheep, deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1947

  Product name: Recombinant human HSP90AB1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-710 aa of BC014485

  Sequence: RALLFIPRRAPFDLFENKKKKNNIKLYVRRVFIMDSCDELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNIVKKCLELFSELAEDKENYKKFYEAFSKNLKLGIHEDSTNRRRLSELLRYHTSQSGDEMTSLSEYVSRMKETQKSIYYITGESKEQVANSAFVERVRKRGFEVVYMTEPIDEYCVQQLKEFDGKSLVSVTKEGLELPEDEEEKKKMEESKAKFENLCKLMKEILDKKVEKVTISNRLVSSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMMAKKHLEINPDHPIVETLRQKAEADKNDKAVKDLVVLLFETALLSSGFSLEDPQTHSNRIYRMIKLGLGIDEDEVAAEEPNAAVPDEIPP

• Full Name: heat shock protein 90kDa alpha (cytosolic), class B member 1
• Calculated Molecular Weight: 723 aa, 83 kDa
• Observed Molecular Weight: 83-90 kDa
• GenBank Accession Number: BC014485
• Gene Symbol: HSP90AB1
• Gene ID (NCBI): 3326
• RRID: AB_2121207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
pig	WB	Autophagy; Induction of autophagy and suppression of type I IFN secretion by CSFV.; Authors - Baoming Xie
	WB	Cell Rep; CGG repeats in the human FMR1 gene regulate mRNA localization and cellular stress in developing neurons; Authors - Carissa L Sirois
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik
human	WB	Anal Chem; Solvent-Induced Protein Precipitation for Drug Target Discovery on the Proteomic Scale.; Authors - Xiaolei Zhang
sheep	IF	PLoS Pathog; A non-enveloped arbovirus released in lysosome-derived extracellular vesicles induces super-infection exclusion.; Authors - Thomas Labadie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Maria (Verified Customer) (07-06-2022)

Very good antibody, we achieved good results.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Fibroblasts - human

FH

Kyosuke (Verified Customer) (06-12-2019)

it works good for WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T