|Positive WB detected in||HeLa cells, multi-cells/tissue, mouse kidney tissue, mouse uterus tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue, mouse skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||HepG2 cells, HeLa cells, A549 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10995-1-AP targets HSP70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||cow, human, mouse, oysters, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HSP70 fusion protein Ag1446|
|Full Name||heat shock 70kDa protein 1A|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC009322|
|Gene ID (NCBI)||3303|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
What is Hsp70/HSP1A?
HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.
FAQs for Hsp70
a. I cannot detect Hsp70 by western blotting
HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.
b. What loading control can I use for cellular stress experiments with Hsp70?
Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.
|Product Specific Protocols|
|WB protocol for HSP70 antibody 10995-1-AP||Download protocol|
|IHC protocol for HSP70 antibody 10995-1-AP||Download protocol|
|IF protocol for HSP70 antibody 10995-1-AP||Download protocol|
|IP protocol for HSP70 antibody 10995-1-AP||Download protocol|
|FC protocol for HSP70 antibody 10995-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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