• Featured Product
  • KD/KO Validated

HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10995-1-AP

Synonyms

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney, rat kidney, A549 cells, mouse brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells, HeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10995-1-AP targets HSP70 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine, C. elegans, ostrich, oyster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP70 fusion protein Ag1446
Full Name heat shock 70kDa protein 1A
Calculated molecular weight 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC009322
Gene symbol HSPA1A
Gene ID (NCBI) 3303
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.

Protocols

Product Specific Protocols
WB protocol for HSP70 antibody 10995-1-APDownload protocol
IHC protocol for HSP70 antibody 10995-1-APDownload protocol
IF protocol for HSP70 antibody 10995-1-APDownload protocol
IP protocol for HSP70 antibody 10995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KO Validated
human,mouseWB,IF

Acta Neuropathol

Distinctive chaperonopathy in skeletal muscle associated with the dominant variant in DNAJB4

Authors - Michio Inoue
humanWB

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao
mouseWB

Neuron

UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
  • KD Validated
human,mouseWB,IHC

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tsimafei (Verified Customer) (12-04-2022)

20µg of the total protein were used. Incubation was done for 2h at room temperature

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: mESCs
HSP70 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:10995-1-AP)
FH

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: ARPE-19
HSP70 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:10995-1-AP)
FH

Luxi (Verified Customer) (11-23-2020)

This antibody works well in my tissue. It binds to the nuclear and cytosol Hsp70s.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:60
  • Cell Tissue Type: embryonic cells