• Featured Product
  • KD/KO Validated

HSP70 Polyclonal antibody

HSP70 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (7)


WB, IP, IF, IHC, CoIP, ELISA, Cell treatment



Cat no : 10995-1-AP


Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Tested Applications

Positive WB detected inA431 cells, human urine exosomes tissue, A549 cells, HeLa cells, Jurkat cells, mouse brain, HEK-293 cells, mouse kidney, rat kidney
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10995-1-AP targets HSP70 in WB, IP, IF, IHC, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, ostrich, oyster, pig, C. elegans, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP70 fusion protein Ag1446
Full Name heat shock 70kDa protein 1A
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC009322
Gene Symbol HSP70
Gene ID (NCBI) 3303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

  1. What is Hsp70/HSP1A?

    HSP1A is a member of the Hsp70 (heat shock protein 70) proteins that act as molecular chaperones ensuring correct protein folding and preventing protein aggregation. Hsp70 protein production is greatly induced by various stress stimuli, including high temperature and toxins. Its expression is often elevated in various cancers.

  2. FAQs for Hsp70

    a. I cannot detect Hsp70 by western blotting

    HSP70s are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Try to always include cell lysate from cells subjected to stress conditions as a positive control.

    b. What loading control can I use for cellular stress experiments with Hsp70?

    Choosing a loading control antibody is an important step in western blotting experimental setup. We highly recommend using more than one loading control while developing new cellular stress assays to ensure that a given treatment does not alter expression of house-keeping genes. Hsp70 has a molecular size of 70 kDa, so we recommend using GAPDH (36 kDa), actin (42 kDa), or tubulin (50-55 kDa). More information on our control antibodies can be found here: https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/.


Product Specific Protocols
WB protocol for HSP70 antibody 10995-1-APDownload protocol
IHC protocol for HSP70 antibody 10995-1-APDownload protocol
IF protocol for HSP70 antibody 10995-1-APDownload protocol
IP protocol for HSP70 antibody 10995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




A cytosolic surveillance mechanism activates the mitochondrial UPR

Authors - F X Reymond Sutandy


PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KO Validated

Mol Cancer

CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.

Authors - Bing Yao


UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.

Authors - Kejing Zhang
  • KD Validated

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Scott (Verified Customer) (05-22-2024)

20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 : 5000
  • Cell Tissue Type: TDP-Q331K Cortex and Spinal Cord Tissue Lysate
HSP70 Antibody Western Blot validation (1 : 5000 dilution) in TDP-Q331K Cortex and Spinal Cord Tissue Lysate (Cat no:10995-1-AP)

Nin (Verified Customer) (01-28-2023)

A very good antibody to probe HSP70 by WB.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse cortex lysate
HSP70 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:10995-1-AP)

Tsimafei (Verified Customer) (12-04-2022)

20µg of the total protein were used. Incubation was done for 2h at room temperature

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: mESCs
HSP70 Antibody Western Blot validation (1:10000 dilution) in mESCs (Cat no:10995-1-AP)

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: ARPE-19
HSP70 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:10995-1-AP)

Luxi (Verified Customer) (11-23-2020)

This antibody works well in my tissue. It binds to the nuclear and cytosol Hsp70s.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:60
  • Cell Tissue Type: embryonic cells