HSPA4L Polyclonal antibody

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  WB analysis using 32468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32468-1-AP (HSPA4L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 32468-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32468-1-AP (HSPA4L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSPA4L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32468-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

APG1, Heat shock 70 kDa protein 4L, Heat shock 70-related protein APG-1, Heat shock protein family H member 3, Heat-shock protein family A member 4-like protein

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, RT-4 cells, U-251 cells
• Positive IHC detected in: human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32468-1-AP targets HSPA4L in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38354

  Product name: Recombinant human HSPA4L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 690-839 aa of NM_001317381.1

  Sequence: MKYMEHEERPKALNDLGKKIQLVMKVIEAYRNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVSEIVAKSKELDNFCNPIIYKPKPKAEVPEDKPKANSEHNGPMDGQSGTETKSDSTKDSSQHTKSSGEMEVD*

• Full Name: heat shock 70kDa protein 4-like
• Calculated Molecular Weight: 95kDa，839aa
• Observed Molecular Weight: 95~100 kDa
• GenBank Accession Number: NM_001317381.1
• Gene Symbol: HSPA4L
• Gene ID (NCBI): 22824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95757
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA4L (Heat shock 70-kDa protein 4-like), also known as APG1 or OSP94, is a member of the heat shock protein 110 family, whose mRNA is strongly expressed in normal human testis and overexpressed in leukemia cells. HSPA4L was also restrictedly expressed in normal tissues and the its product was capable of eliciting a humoral immune response likely induced by its high-level expression in leukemia cells. Up-regulation of the Hspa4l gene in kidney and renal cell lines by osmotic stress suggested that Hspa4l may function to enable the kidney to compensate for osmotic stress present within the kidney (PMID: 16923965, 17588478).