Hsc70 Polyclonal antibody

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  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10654-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10654-1-AP (Hsc70 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10654-1-AP

  IP result of anti-Hsc70 (IP:10654-1-AP, 4ug; Detection:10654-1-AP 1:2000) with HEK-293 cells lysate 1640 ug.

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  IHC staining of human renal cell carcinoma using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10654-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10654-1-AP (Hsc70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10654-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using Hsc70 antibody (10654-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

Hsc70 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10654-1-AP

Publications(76)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

HSPA8, HSPA10, HSP73, Heat shock protein family A member 8, Heat shock cognate 71 kDa protein

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Tested Applications

• Positive WB detected in: HEK-293 cells, MCF-7 cells, PC-12 cells, HeLa cells, NIH/3T3 cells, U-87 MG cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 59 publications below
• IHC: See 5 publications below
• IF: See 14 publications below
• IP: See 7 publications below
• CoIP: See 7 publications below

Product Information

10654-1-AP targets Hsc70 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Hsc70 fusion protein Ag1018
• Full Name: heat shock 70kDa protein 8
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007276
• Gene Symbol: HSC70
• Gene ID (NCBI): 3312
• RRID: AB_2120153
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11142
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease (21832061). In addition, this antibody is most likely able to recognize Hsp70.

Publications

Species	Application	Title
human	WB	Nature; A dominant autoinflammatory disease caused by non-cleavable variants of RIPK1.; Authors - Panfeng Tao
		J Extracell Vesicles; Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk.; Authors - Tsukasa Kadota
mouse	WB	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
human	WB	Genes Dev; Chaperone-mediated autophagy degrades mutant p53.; Authors - Vakifahmetoglu-Norberg Helin H
mouse	WB	Redox Biol; WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury; Authors - Zebin Deng
human	WB,IF	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

Hsc70 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10654-1-AP)

FH

Mayur (Verified Customer) (04-30-2019)

Great antibody. Detects the HSC70 expression in the H4 cells very clearly.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4

Hsc70 Antibody Western Blot, validation (1:1000 dilution) in H4 (Cat no:10654-1-AP)