|Positive WB detected in||HEK-293 cells, A431 cells, C6 cells, rat brain tissue, COLO 320 cells, HeLa cells, MCF-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human ovary tumor tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10654-1-AP targets Hsc70 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Hsc70 fusion protein Ag1018|
|Full Name||heat shock 70kDa protein 8|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||70-73 kDa|
|GenBank accession number||BC007276|
|Gene ID (NCBI)||3312|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (21832061)
|Product Specific Protocols|
|WB protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IHC protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IF protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IP protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Chaperone-mediated autophagy is involved in the execution of ferroptosis.
Cell Death Dis
Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI.
Cell Stress Chaperones
PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.
Biochem Biophys Res Commun
Exosomes derived from breast cancer lung metastasis subpopulations promote tumor self-seeding.
J Agric Food Chem
A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.
hERG Potassium Channel Blockage by Scorpion Toxin BmKKx2 Enhances Erythroid Differentiation of Human Leukemia Cells K562.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.
Mayur (Verified Customer) (04-30-2019)
Great antibody. Detects the HSC70 expression in the H4 cells very clearly.