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  • KD/KO Validated

Hsc70 Polyclonal antibody

Hsc70 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10654-1-AP

Synonyms

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, Heat shock cognate protein 71 kDa, HSC54, HSC70, HSC71, HSP71, HSP73, HSP7C, HSPA10, HSPA8, LAP1



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, PC-12 cells, HeLa cells, NIH/3T3 cells, U-87 MG cells, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary tumor tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10654-1-AP targets Hsc70 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Hsc70 fusion protein Ag1018
Full Name heat shock 70kDa protein 8
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007276
Gene Symbol HSPA8
Gene ID (NCBI) 3312
RRIDAB_2120153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease (21832061). In addition, this antibody is most likely able to recognize Hsp70.

Protocols

Product Specific Protocols
WB protocol for Hsc70 antibody 10654-1-APDownload protocol
IHC protocol for Hsc70 antibody 10654-1-APDownload protocol
IF protocol for Hsc70 antibody 10654-1-APDownload protocol
IP protocol for Hsc70 antibody 10654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A dominant autoinflammatory disease caused by non-cleavable variants of RIPK1.

Authors - Panfeng Tao

J Extracell Vesicles

Human bronchial epithelial cell-derived extracellular vesicle therapy for pulmonary fibrosis via inhibition of TGF-β-WNT crosstalk.

Authors - Tsukasa Kadota
mouseWB

Cell Rep Med

Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing

Authors - Dongming Lv
humanWB

Genes Dev

Chaperone-mediated autophagy degrades mutant p53.

Authors - Vakifahmetoglu-Norberg Helin H
mouseWB

Redox Biol

WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury

Authors - Zebin Deng
humanWB,IF

Redox Biol

Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.

Authors - Caiying Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart
Hsc70 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:10654-1-AP)
FH

Mayur (Verified Customer) (04-30-2019)

Great antibody. Detects the HSC70 expression in the H4 cells very clearly.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H4
Hsc70 Antibody Western Blot, validation (1:1000 dilution) in H4 (Cat no:10654-1-AP)