|Positive WB detected in||HEK-293 cells, A431 cells, C6 cells, rat brain tissue, COLO 320 cells, HeLa cells, MCF-7 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human ovary tumor tissue, human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:400-1:1600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10654-1-AP targets Hsc70 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Hsc70 fusion protein Ag1018|
|Full Name||heat shock 70kDa protein 8|
|Calculated molecular weight||70 kDa|
|Observed molecular weight||70-73 kDa|
|GenBank accession number||BC007276|
|Gene ID (NCBI)||3312|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson’s disease, indicating its implication in neurodegenerative disease. (21832061)
|Product Specific Protocols|
|WB protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IHC protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IF protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|IP protocol for Hsc70 antibody 10654-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
I had a very strong and specific Hsc70 band at ~70 kDa. Definitely one of the best antibodies I have used for Hsp70 family proteins.
Mayur (Verified Customer) (04-30-2019)
Great antibody. Detects the HSC70 expression in the H4 cells very clearly.