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  • KD/KO Validated

HUS1 Polyclonal antibody

HUS1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG







Cat no : 11223-1-AP


Checkpoint protein HUS1, hHUS1, HUS1

Tested Applications

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11223-1-AP targets HUS1 in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HUS1 fusion protein Ag1744
Full Name HUS1 checkpoint homolog (S. pombe)
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC007013
Gene symbol HUS1
Gene ID (NCBI) 3364
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].


Product Specific Protocols
WB protocol for HUS1 antibody 11223-1-APDownload protocol
IHC protocol for HUS1 antibody 11223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen

J Mol Cell Biol

SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.

Authors - Xiaoli Xu

Mol Carcinog

HUS1 checkpoint clamp component (HUS1) is a potential tumor suppressor in primary hepatocellular carcinoma.

Authors - Zi-Qi Zhou
  • KD Validated

Am J Physiol Cell Physiol

Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.

Authors - Rongyi Shi

Dis Markers

Discovery and Validation of Novel Methylation Markers in Helicobacter pylori-Associated Gastric Cancer.

Authors - Huan Wang