"Histone-H3 Antibodies" Comparison
View side-by-side comparison of Histone-H3 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||HEK-293 cells, A549 cells, mouse skeletal muscle tissue, mouse liver tissue, mouse brain tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue|
|Positive IF detected in||HEK-293 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Arabidopsis, Bovine, canine, chicken, fish, frog, goat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone-H3 fusion protein Ag10644|
|Full Name||histone cluster 2, H3a|
|Calculated molecular weight||136 aa, 15 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC015544|
|Gene ID (NCBI)||333932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
|Product Specific Protocols|
|WB protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IHC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IF protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|FC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|Click here to view our Standard Protocols|
The molecular basis of tight nuclear tethering and inactivation of cGAS.
A phase-separated nuclear GBPL circuit controls immunity in plants.
Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.
N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.
Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation.
Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Veda (Verified Customer) (04-29-2022)
IF staining test in cryo sections of skin. It does not seem to work. Maybe it only stains the nuclear membrane.
Pradeep (Verified Customer) (09-19-2020)
Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4
Tanusree (Verified Customer) (12-03-2019)
The antibody works great in Western blotting analysis.
Ruiting (Verified Customer) (11-11-2019)
I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting
Robert (Verified Customer) (07-18-2019)
The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.