|Positive WB detected in||HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse liver tissue, A549 cells|
|Positive IHC detected in||human skin cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Arabidopsis, Bovine, fish, frog, human, monkey, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone-H3 fusion protein Ag10644|
|Full Name||histone cluster 2, H3a|
|Calculated molecular weight||136 aa, 15 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC015544|
|Gene ID (NCBI)||333932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
|Product Specific Protocols|
|WB protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IHC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IF protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|FC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pradeep (Verified Customer) (09-19-2020)
Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4
Tanusree (Verified Customer) (12-03-2019)
The antibody works great in Western blotting analysis.
Ruiting (Verified Customer) (11-11-2019)
I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting
Robert (Verified Customer) (07-18-2019)
The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.