Histone-H3 Polyclonal antibody

Histone-H3 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, FC, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 17168-1-AP

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o


"Histone-H3 Antibodies" Comparison

View side-by-side comparison of Histone-H3 antibodies from other vendors to find the one that best suits your research needs.
  • WB analysis of HEK-293 using 17168-1-AP

    WB analysis of HEK-293 using 17168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 17168-1-AP

    FC experiment of HeLa using 17168-1-AP

    1X10^6 HeLa cells were stained with 0.20ug Histone-H3 antibody (17168-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

  • IHC staining of human breast cancer using 17168-1-AP

    IHC staining of human breast cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skin cancer using 17168-1-AP

    IHC staining of human skin cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HEK-293 using 17168-1-AP

    IF Staining of HEK-293 using 17168-1-AP

    Immunofluorescent analysis of HEK-293 cells using 17168-1-AP (Histone-H3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.


Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse liver tissue, A549 cells
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity Arabidopsis, Bovine, canine, fish, frog, human, monkey, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone-H3 fusion protein Ag10644
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC015544
Gene symbol HIST2H3A
Gene ID (NCBI) 333932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocols

Product Specific Protocols
WB protocol for Histone-H3 antibody 17168-1-APDownload protocol
IHC protocol for Histone-H3 antibody 17168-1-APDownload protocol
IF protocol for Histone-H3 antibody 17168-1-APDownload protocol
FC protocol for Histone-H3 antibody 17168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biomed Pharmacother

CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.

Authors - Xuezhi Yang
humanWB

Theranostics

Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming.

Authors - Yujing Tan
humanWB

Free Radic Biol Med

5-(3,4-Difluorophenyl)-3-(6-methylpyridin-3-yl)-1,2,4-oxadiazole (DDO-7263), a Novel Nrf2 Activator Targeting Brain Tissue, Protects Against MPTP-induced Subacute Parkinson's Disease in Mice by Inhibiting the NLRP3 Inflammasome and Protects PC12 Cells Against Oxidative Stress.

Authors - Li-Li Xu
ratWB

Iran J Basic Med Sci

Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.

Authors - Yeying Wang
mouseWB

Mol Immunol

Blockade of YAP alleviates hepatic fibrosis through accelerating apoptosis and reversion of activated hepatic stellate cells.

Authors - Hai-Xia Yu
humanWB

FEBS J

Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.

Authors - Yi-Xuan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pradeep (Verified Customer) (09-19-2020)

Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart Tissue
FH

Tanusree (Verified Customer) (12-03-2019)

The antibody works great in Western blotting analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Ruiting (Verified Customer) (11-11-2019)

I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse testis and brains
FH

Robert (Verified Customer) (07-18-2019)

The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
Histone-H3 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:17168-1-AP)