"Histone-H3 Antibodies" Comparison
View side-by-side comparison of Histone-H3 antibodies from other vendors to find the one that best suits your research needs.
|Positive WB detected in||HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse liver tissue, A549 cells|
|Positive IHC detected in||human skin cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Arabidopsis, Bovine, canine, fish, frog, human, monkey, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone-H3 fusion protein Ag10644|
|Full Name||histone cluster 2, H3a|
|Calculated molecular weight||136 aa, 15 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC015544|
|Gene ID (NCBI)||333932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
|Product Specific Protocols|
|WB protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IHC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|IF protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|FC protocol for Histone-H3 antibody 17168-1-AP||Download protocol|
|Click here to view our Standard Protocols|
CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.
Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming.
Free Radic Biol Med
5-(3,4-Difluorophenyl)-3-(6-methylpyridin-3-yl)-1,2,4-oxadiazole (DDO-7263), a Novel Nrf2 Activator Targeting Brain Tissue, Protects Against MPTP-induced Subacute Parkinson's Disease in Mice by Inhibiting the NLRP3 Inflammasome and Protects PC12 Cells Against Oxidative Stress.
Iran J Basic Med Sci
Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.
Blockade of YAP alleviates hepatic fibrosis through accelerating apoptosis and reversion of activated hepatic stellate cells.
Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Pradeep (Verified Customer) (09-19-2020)
Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4
Tanusree (Verified Customer) (12-03-2019)
The antibody works great in Western blotting analysis.
Ruiting (Verified Customer) (11-11-2019)
I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting
Robert (Verified Customer) (07-18-2019)
The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.