Cardinal Red™-conjugated Histone-H3 Polyclonal antibody

Histone-H3 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

Cardinal Red™ Fluorescent Dye

Cat no : CR-17168

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o

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Validation Data Gallery

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  FC experiment of HeLa using CR-17168

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human Histone-H3 (CR-17168) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CR-17168 is Histone-H3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Histone-H3 fusion protein Ag10644
• Full Name: histone cluster 2, H3a
• Calculated molecular weight: 136 aa, 15 kDa
• Observed molecular weight: 15-17 kDa
• GenBank accession number: BC015544
• Gene symbol: HIST2H3A
• Gene ID (NCBI): 333932
• Conjugate: Cardinal Red™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 592 nm / 611 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.