Recombinant Human ACAN protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg2994

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Validation Data Gallery

• 

  Purity of Recombinant Human ACAN was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ACAN protein Val20-Arg675 (Accession# P16112-1) with a rabbit IgG Fc tag at the C-terminus
• GeneID: 176
• Accession: P16112-1
• PredictedSize: 98.6 kDa
• SDS-PAGE: 110-130 kDa, reducing (R) condition
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ACAN, or Aggrecan, is a key component of the extracellular matrix in cartilaginous tissues. Acan is present in cartilage, intervertebral disc, brain, heart, and aorta (PMID: 35385326). Its core protein contains three glubular domains and two glycosaminoglycan-attachment domains. These domains play various roles to maintain cartilage structure and function.

References:

1. Watanabe H. (2022). Am J Physiol Cell Physiol. 322(5):C967-C976.
2. Watanabe H. et al. (1998). J Biochem. 124(4):687-93.