Recombinant Human ADAM17 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

C-rFc

Activity

not tested

Cat no : Eg4732

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Validation Data Gallery

• 

  Purity of Recombinant Human ADAM17 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE; >90 %, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ADAM17 protein Phe470-Leu570 (Accession# P78536-1) with a rabbit IgG Fc tag at the C-terminus
• GeneID: 6868
• Accession: P78536-1
• PredictedSize: 37.2 kDa
• SDS-PAGE: 48-58 kDa, reducing (R) condition
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor. ADAM17 is also named CSVP, TACE. The full-length protein has 9 glycosylation sites, a signal peptide, propeptide, and 2 isoforms produced by alternative splicing.

References:

1. P M McGowan. et al. (2008). Ann Oncol. 19(6):1075-1081.
2. Patricia M McGowan. et al.(2007). Clin Cancer Res.13(8):2335-2343.
3. Bin-Bing S Zhou. et al. (2006). Cancer Cell. 10(1):39-50.