Human APOE ELISA Kit

Sensitivity

29.4 pg/mL

Range

78.1-5000 pg/mL

Reactivity

Human

Cat no : KE00156

Synonyms

AD2, Apo E, APOE, apolipoprotein E, LPG



Product Information

KE00156 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOE ELISA kit is to be used to detect and quantify protein levels of endogenous APOE. The assay recognizes human APOE. An antibody specific for APOE has been pre-coated onto the microwells. The APOE protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for APOE is added to detect the captured APOE protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human APOE ELISA Kit
Tests 1 X 96 well plate
Sample type Human serum, Human plasma,Urine, Saliva, Human cerebrospinal fluid
Assay type Sandwich
Sensitivity 29.4 pg/mL
Range 78.1-5000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human serum100%81%-118%
Urine113%99%-123%
Saliva105%77%-125%
Human cerebrospinal fluid81%73%-96%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,811.288.63.2
220776.023.93.1
320291.07.42.5

InterAssay

Samplenmean ( pg/mL)SDCV%
1242,846.8200.87.1
224729.622.33.1
324283.911.13.9

Background Information

APOE (apolipoprotein E) is a plasma protein implicated in lipid metabolism. It is produced predominantly by hepatocytes, macrophages, and neural cells. In the CNS, APOE is the major extracellular lipid carrier and plays a key role in neuronal protection/repair after injury. APOE is polymorphic, with three major alleles: APOE2 (cys112, cys158), APOE3 (cys112, arg158), and APOE4 (arg112, arg158). APOE2 plays a protective role against both Alzheimer's (AD) and heart disease. APOE4 confers a higher risk for atherosclerosis and Alzheimer's disease. Analysis of cerebrospinal fluid (CSF) and plasma APOE protein levels in AD patients had been performed to examine its association with AD pathology. In old age, high plasma APOE levels precede an increase of circulating CRP and strongly associates with cardiovascular mortality.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AD2, Apo E, APOE, apolipoprotein E, LPG