Recombinant Human ATP1B3 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4947

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Validation Data Gallery

• 

  Purity of Recombinant Human ATP1B3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human ATP1B3 protein Thr57-Ala279 (Accession# P54709-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 483
• Accession: P54709-1
• PredictedSize: 52.2 kDa
• SDS-PAGE: 52-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells.

References:

1. Zhang, Shu-Hong et al. The Journal of biological chemistry vol. 294,16 (2019): 6375-6386. 
2. Lian, Wei-Nan et al. Journal of cell science vol. 119,Pt 1 (2006): 11-22. 
3. Chiampanichayakul, S et al. Tissue antigens vol. 68,6 (2006): 509-17.