Human AXL ELISA Kit
Cat no : KE00807
Synonyms
EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO
Validation Data Gallery
Product Information
KE00807 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human AXL ELISA kit is to be used to detect and quantify protein levels of endogenous human AXL. The assay recognizes human AXL. An antibody specific for human AXL has been pre-coated onto the microwells. The human AXL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human AXL is added to detect the captured human AXL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human AXL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate, CSF |
| Assay type | Sandwich |
| Sensitivity | 7.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 558 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 102% | 89%-124% |
| Urine | 94% | 83%-115% |
| Cell culture supernatant | 97% | 88%-112% |
| Cell lysate | 95% | 88%-105% |
| CSF | 90% | 88%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 996.5 | 48.2 | 4.8 |
| 2 | 8 | 230.6 | 15.4 | 6.7 |
| 3 | 8 | 111.2 | 3.9 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 972.4 | 51.7 | 5.3 |
| 2 | 16 | 238.5 | 17.3 | 7.3 |
| 3 | 16 | 120.5 | 10.4 | 8.6 |
Background Information
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl, and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains, and one protein kinase domain. Axl mRNA is widely expressed with the highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration, and differentiation. Endogenous Axl is auto-phosphorylated and reacts with a tyrosine-phosphorylated protein of 140 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO |