Human Alpha-1-microglobulin ELISA Kit
Cat no : KE01027
Synonyms
AMBP, A1M, Alpha 1 microglobulin, Alpha 1 microglycoprotein, Alpha 1-microglobulin
Validation Data Gallery
Product Information
KE01027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Alpha-1-microglobulin ELISA kit is to be used to detect and quantify protein levels of endogenous human Alpha-1-microglobulin. The assay recognizes human Alpha-1-microglobulin. An antibody specific for human Alpha-1-microglobulin has been pre-coated onto the microwells. The human Alpha-1-microglobulin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Alpha-1-microglobulin is added to detect the captured human Alpha-1-microglobulin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Alpha-1-microglobulin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 259 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 87%-107% |
| Cell culture supernatant | 106% | 94%-119% |
| Urine | 98% | 86%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.26 | 0.17 | 3.30 |
| 2 | 8 | 1.53 | 0.04 | 2.62 |
| 3 | 8 | 0.52 | 0.03 | 6.19 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.00 | 0.31 | 6.12 |
| 2 | 16 | 1.47 | 0.08 | 5.12 |
| 3 | 16 | 0.49 | 0.04 | 7.73 |
Background Information
Alpha-1-microglobulin (A1M) is also named as HI30, Protein AMBP, Bikunin, Trypstatin and Inter-alpha-trypsin inhibitor light chain (ITI-LC). A1M belongs to the lipocalin protein family, a group of structural proteins with a similar one-domain fold that are found in bacteria, plants and animals. Alpha 1 microglobulin is a 27-30 kDa glycoprotein, present in various body fluids. It belongs to the lipocalin superfamily of hydrophobic ligand-binding proteins forming an internal ligand-binding pocket. The protein acts as a mediator of bacterial adhesion to polymer surfaces and is involved in inhibiting renal lithogenesis. A1M is recognized as a physiological antioxidant with powerful cell- and tissue-protective properties. Whereas A1M is a heme and radical scavenger, and a reductase, bikunin is a structural component of extracellular matrix and has protease inhibitor and anti-inflammatory properties. In humans, the majority of A1M is synthesized in the liver, but smaller quantities are also expressed in most other cells in the body. In the blood, equal quantities of two forms of A1M can be found: a free monomeric form and a covalent high-molecular weight complex bound to immunoglobulin A, albumin and prothrombin. Endogenous A1M has also been described to be localized ubiquitously in the dermal and epidermal layers of skin, and in syncytiothrophoblasts, monocytes/macrophages, vascular endothelium and extracellular matrix of placental tissue and extracellular matrix.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AMBP, A1M, Alpha 1 microglobulin, Alpha 1 microglycoprotein, Alpha 1-microglobulin |