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Recombinant Human APOE protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0448

Validation Data Gallery

  • Purity of Recombinant Human APOE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human APOE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human APOE protein Lys19-His317 (Accession# P02649) with a His tag at the C-terminus.
GeneID 348
Accession P02649
PredictedSize 38 kDa
SDS-PAGE 34-36 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Apolipoprotein E (APOE) is a core component of plasma lipoproteins and is involved in a variety of biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immune regulation, and neuronal growth and repair.The ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Mouse APOE, like ApoE4, contains equivalents of Arg-112 and Glu-255 but lacks the critical Arg-61 equivalent. APOE has heparin-binding activity and binds to heparan sulfate proteoglycans on the cell surface, a property that facilitates cellular capture and receptor-mediated uptake of APOE-containing lipoproteins.

References:

1. Teng E.,et al. (2015). Dement Geriatr Cogn Disord.39(3-4):154-66.
2. Raffai R.L.,et al. (2001). Proc Natl Acad Sci U S A.98(20):11587-11591.
3. Mooijaart SP,et al. (2006). PLoS Med.3(6):e176.
4. Maloney B,et al. (2007).J Neurochem.103(3):1237-1257.