Recombinant Human Biglycan protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2972

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Validation Data Gallery

• 

  Purity of Recombinant Human Biglycan was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Biglycan protein Leu17-Lys368 (Accession# P21810) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 633
• Accession: P21810
• PredictedSize: 65.8 kDa
• SDS-PAGE: 68-100 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family. It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound. The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein. Biglycan is a ubiquitous component of the connective tissue matrix and may act as a signaling molecule. Upregulation of biglycan has been reported in multiple types of solid cancer.

References:

1.Schaefer L. et al. (2008). J Biol Chem. 283(31):21305-21309.
2.Bianco P. et al. (1990). J Histochem Cytochem. 38(11):1549-1563.
3.Iozzo RV. (1999). J Biol Chem. 274(27):18843-18846.
4.Nastase MV. et al. (2012). J Histochem Cytochem. 60(12):963-975.
5.Zhao SF. et al. (2020). Oncol Lett. 19(3):1673-1682.