Speedy™ Human CCL7/MCP-3 One-Step ELISA Kit
Cat no : SE50226
Synonyms
CCL7, CCL7, MCP3, C C motif chemokine 7, CCL 7, CCL-7
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CCL7/MCP-3 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 31.25 -2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6354 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 80% | 80%-81% |
| Cell culture supernatant | 102% | 98%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,040.6 | 13.3 | 1.3 |
| 2 | 8 | 250.8 | 3.6 | 1.4 |
| 3 | 8 | 127.6 | 3.6 | 2.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,039.5 | 19.4 | 1.9 |
| 2 | 16 | 252.5 | 4.6 | 1.8 |
| 3 | 16 | 128.4 | 2.9 | 2.3 |
Background Information
The chemokine CCL7 (MCP3) is a chemotactic factor and potent attractant of monocytes firstly characterized from the culture supernatants of MG-63 osteosarcoma cells. CCL7 is known to promote the recruitment of many innate immune cell types including monocytes and neutrophils to sites of bacterial and viral infection and eosinophils and basophils to sites of allergic inflammation. CCL7 is expressed at low levels in endothelial cells, fibroblasts and mononuclear cells and upregulated by various stimuli including viruses, type I or type II interferons (IFNs). CCL7 (MCP-3) mediates effects on a host of innate and adaptive immune cell types through binding to numerous receptors including CCR1, CCR2, CCR3, CCR5, and CCR10. Various studies have shown that tumor cells and stromal cells also produce high levels of CCL7, while the specific response element and signaling pathways involved are not entirely clear.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL7, CCL7, MCP3, C C motif chemokine 7, CCL 7, CCL-7 |