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Recombinant Human CD48 protein (His tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His tag

Activity

EC50: 10-41 ng/mL

Cat no : Eg1005

Validation Data Gallery

  • Purity of Recombinant Human CD48 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD48 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CD48 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD244 (rFc tag) with a linear range of 10-41 ng/mL.

    Immobilized Human CD48 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD244 (rFc tag) with a linear range of 10-41 ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human CD48 (His tag) at 2 μg/mL (100 μL/well) can bind Human CD244 (rFc tag) with a linear range of 10-41 ng/mL.
Expression HEK293-derived Human CD48 protein Gln27-Ser220 (Accession# P09326) with a His tag at the C-terminus.
GeneID 962
Accession P09326
PredictedSize 23.4 kDa
SDS-PAGE
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD48, also known as BLAST-1 or BCM-1, is a member of the signaling lymphocyte activation molecule (SLAM) family and is involved in immune cell adhesion and activation. It is a glycosylphosphatidylinositol (GPI) -anchored cell surface glycoprotein that interacts with various cell surface receptors, including CD244 (2B4) and CD2. CD48 plays a crucial role in regulating immune responses by mediating cell-cell interactions and signaling pathways that influence T cell activation, NK cell function, and the formation of immune synapses. Soluble CD48 (sCD48) is detected in human serum and plasma. sCD48 may serve as a potential therapeutic agent for inflammatory diseases by modulating immune cell activation and cytokine signaling.

References:

1. McArdel, Shannon L et al. Clinical immunology (Orlando, Fla.) vol. 164 (2016): 10-20.
2. Smith, G M et al. Journal of clinical immunology vol. 17,6 (1997): 502-509.
3. Breuer, Oded et al. Journal of immunology research vol. (2018): 4236263.