Speedy™ Human CD5 One-Step ELISA Kit
Cat no : SE50158
Synonyms
CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, T1
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD5 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 921 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 94%-110% |
| Cell lysate | 103% | 96%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,044.1 | 36.2 | 3.5 |
| 2 | 8 | 259.5 | 12.5 | 4.8 |
| 3 | 8 | 139.6 | 7.9 | 5.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,093.7 | 85.4 | 7.8 |
| 2 | 16 | 257.4 | 10.8 | 4.2 |
| 3 | 16 | 141.0 | 7.4 | 5.2 |
Background Information
CD5 antigen (CD5, also known as Ly-1, Ly-A, Ly-12, and Lyt-1) is a pan-T cell marker and a negative regulator of TCR and BCR signaling, which increases expression during T-cell development in the thymus. It is associated with the B cell receptor (BCR) complex and is a substrate for BCR-induced tyrosine kinase activity. In addition to membrane-bound CD5, the circulating soluble form of CD5 (sCD5) has been shown in sera resulting from proteolytic cleavage following lymphocyte activation. The expression level of sCD5 is associated with the development of autoimmune and inflammatory disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, Lymphocyte antigen T1/Leu-1, T1 |