Recombinant Human CD6 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2079

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Validation Data Gallery

• 

  Purity of Recombinant Human CD6 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD6 protein His18-Glu398 (Accession# P30203-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 923
• Accession: P30203-1
• PredictedSize: 66.9 kDa
• SDS-PAGE: 80-120 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD6 is a type I transmembrane glycoprotein of 105-130 kDa that belongs to the scavenger receptor cysteine-rich superfamily. It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction. CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain. CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

References:

1. Vanesa Gabriela Martínez, et al. (2011) Pharmacol Rev. 63(4):967-1000.
2. Lucía Aragón-Serrano, et al. (2023) Int J Mol Sci. 24(24):17510.
3. Lizette Bonet, et al. (2013) FEBS Lett. 587(14):2205-13.
4. A Aruffo, et al. (1991) J Exp Med. 174(4):949-52.
5. Monika Braun, et al. (2011) J Innate Immun. 3(4):420-34.