Recombinant Human CD8a protein (His Tag)

Species

Human

Purity

>70 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0819

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Validation Data Gallery

• 

  Purity of Recombinant Human CD8a was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >70 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD8a protein Ser22-Asp182 (Accession# P01732-1) with a His tag at the C-terminus.
• GeneID: 925
• Accession: P01732-1
• PredictedSize: 18.7 kDa
• SDS-PAGE: 27-30 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β. CD8a (CD8α) is a subunit of this complex. CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells. CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation. During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells.

References:

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6. Zúñiga-Pflücker, J C et al. The Journal of experimental medicine vol. 171,2 (1990): 427-37.
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