Recombinant Human CD9 protein (hFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

hFc Tag

Activity

not tested

Cat no : Eg1080

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Validation Data Gallery

• 

  Purity of Recombinant Human CD9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CD9 protein Ser112-Ile195 (Accession# P21926) with a human IgG1 Fc tag at the N-terminus.
• GeneID: 928
• Accession: P21926
• PredictedSize: 36.5 kDa
• SDS-PAGE: 34-36 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells. It is often used as a mesenchymal stem cell marker.

References:

1.Reyes R.et al.(2018) Front Immunol.9:863.
2.Brosseau C.et al.(2018) Front Immunol.9:2316.
3.Yang XH.et al.(2006) J Biol Chem.281:12976-85.
4.Berditchevski F.et al.(1999) J Cell Biol.146:477-92.
5.Hwang JR.et al.(2012) Carcinogenesis.33:77-83.