Recombinant Human CLEC5A protein (rFc Tag)(HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg5030

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Validation Data Gallery

• 

  Purity of Recombinant Human CLEC5A was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  The purity of Human CLEC5A was greater than 90% as determined by SEC-HPLC.

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Product Information

• Purity: >90 %, SDS-PAGE; >90%, SEC-HPLC
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CLEC5A protein Pro28-Lys188 (Accession# Q9NY25-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 23601
• Accession: Q9NY25-1
• PredictedSize: 45.6 kDa
• SDS-PAGE: 50-70 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CLEC5A is critical for dengue-virus-induced lethal disease, and interacts with the dengue virion directly and thereby brings about DAP12 phosphorylation. CLEC5A is critical to regulate NLRP3 inflammasome activation in DV infection and that the blockade of CLEC5A would be helpful to alleviate clinical symptoms in dengue patients. CLEC5A has a pivotal function in the activation of multiple aspects of innate immunity against bacterial invasion.

References:

1. Chen ST, et al. (2008). Nature. 453(7195):672-6.
2. Wu MF, et al. (2013). Blood. 121(1):95-106.
3. Chen ST, et al. (2017). Nat Commun. 8(1):299.