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Recombinant Human COL18A1 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0254

Validation Data Gallery

  • Purity of Recombinant Human COL18A1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human COL18A1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human COL18A1 protein His1572-Lys1754 (Accession# P39060-3) with a His tag at the C-terminus.
GeneID 80781
Accession P39060-3
PredictedSize 24.4 kDa
SDS-PAGE 25-28 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Collagen XVIII is a heparan sulphate proteoglycan, which in Western blotting occurs as a smear with a molecular weight of approximately 300 kDa, when isolated from human kidney or placenta or from chick vitreous body. Heparinase treatment reduces the molecule size to a protein core of 180 kDa. Collagen XVIII has several potential glycosaminoglycan (GAG)attachmentsites, three of which, located in the NC11, NC9 and NC8 domains, are conserved between mouse, human, Xenopus and chick samples and have also been found to be glycosylated in recombinant chick collagen XVIII produced in chick meningeal cells.

References:

1. Dong, Sucai et al. The Journal of biological chemistry vol. 278,3 (2003): 1700-7.
2. Seppinen, Lotta, and Taina Pihlajaniemi. Matrix biology : journal of the International Society for Matrix Biology vol. 30,2 (2011): 83-92.