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Recombinant human CPE protein (rFc Tag)

Species

Human

Purity

>85 %, SDS-PAGE

Tag

C-rFc

Activity

not tested

Cat no : Eg2989

Validation Data Gallery

  • Purity of Recombinant Human CPE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) condition and stained using Coomassie blue.

    Purity of Recombinant Human CPE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) condition and stained using Coomassie blue.

Product Information

Purity >85 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CPE protein Glu26-Ser453 (Accession# P16870-1) with a rabbit IgG Fc tag at the C-terminus
GeneID 1363
Accession P16870-1
PredictedSize 73.9 kDa
SDS-PAGE 68-80 kDa, reducing (R) condition
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CPE is a multifunctional protein harboring both enzymatic and nonenzymatic functions, largely expressed in endocrine cells and peptidergic neurons in the nervous system. Full-length CPE (F-CPE) protein is an inhibitor of Wnt/β-catenin signaling, which disrupts disheveled-induced signalosomes that are important for transducing the Wnt signal and reduces β-catenin protein levels and activity (PMID: 22824791). Defects in the gene encoding carboxypeptidase E (CPE) in either mouse or human lead to multiple endocrine disorders, including obesity and diabetes (PMID: 16219026).

References:

1. Skalka N. et al. (2013). Oncogene. 32(23):2836-47.
2. Zhu X. et al. (2005). J Neurochem. 95(5):1351-62.