Recombinant Human CRTAM/CD355 protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1541

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Validation Data Gallery

• 

  Purity of Recombinant Human CRTAM was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human CRTAM protein Ser18-Ser286 (Accession# O95727-1) with a His tag at the C-terminus.
• GeneID: 56253
• Accession: O95727-1
• PredictedSize: 31.0 kDa
• SDS-PAGE: 40-68 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion. CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes. It also regulates CD8+ T cell retention within the lymph node and eventually effector function. The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa.

References:

1.Yeh JH, et al. (2008). Cell. 132(5):846-59.
2.Garay E, et al. (2010). J Cell Biochem. 111(1):111-22.
3.Takeuchi A, et al. (2016). J Exp Med. 213(1):123-38.
4.Takeuchi A, et al. (2009). J Immunol. 183(7):4220-8.
5.Patiño-Lopez G, et al. (2006). J Neuroimmunol. 171(1-2):145-55.