Speedy™ Human CTLA-4/CD152 One-Step ELISA Kit
Cat no : SE50106
Synonyms
CTLA4, CD152, CELIAC3, CTLA 4, Cytotoxic T-lymphocyte protein 4
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CTLA-4/CD152 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plsama, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1493 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 79%-109% |
| Cell culture supernatant | 88% | 79%-97% |
| Urine | 83% | 70%-100% |
| Cell lysate | 89% | 72%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 725.7 | 23.3 | 3.2 |
| 2 | 8 | 184.8 | 4.0 | 2.2 |
| 3 | 8 | 101.8 | 3.0 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 703.0 | 55.2 | 7.9 |
| 2 | 16 | 175.6 | 10.2 | 5.8 |
| 3 | 16 | 98.2 | 4.6 | 4.7 |
Background Information
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CTLA4, CD152, CELIAC3, CTLA 4, Cytotoxic T-lymphocyte protein 4 |