Recombinant Human DEFB119 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4054

---

Validation Data Gallery

• 

  Purity of Recombinant Human DEFB119 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human DEFB119 protein Lys22-Pro84 (Accession# Q8N690-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 245932
• Accession: Q8N690-1
• PredictedSize: 33.8 kDa
• SDS-PAGE: 35-42 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

DEFB119, also known as defensin beta 119, is a protein that belongs to the beta subfamily of defensins. These are antimicrobial peptides that play a critical role in protecting tissues and organs from infection by a variety of microorganisms. The DEFB119 gene is located on the long arm of chromosome 20 and is part of a cluster of other beta-defensin genes. This protein has been shown to have antibacterial activity and is involved in the innate immune response, particularly in the defence against bacteria, including both gram-negative and gram-positive bacteria, and fungi.

References:

1.Li X, et al. (2022) Cell Rep Med. 3(12):100825.
2.Holly MK, et al. (2017) Annu Rev Virol. 4(1):369-391.
3.Radhakrishnan Y, et al. (2005) Genes Immun. 6(3):203-10.