Recombinant Human DSG3 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2948

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Validation Data Gallery

• 

  Purity of Recombinant Human DSG3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human DSG3 protein Glu50-Arg615 (Accession# P32926) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 1830
• Accession: P32926
• PredictedSize: 89.0 kDa
• SDS-PAGE: 80-110 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

DSG3 (Desmoglein 3) is a member of the calcineurin superfamily. DSG3 is a calcium-binding transmembrane glycoprotein preferentially found in bridge grains of squamous epithelial keratinocytes. Both deletion and up-regulation of DSG3 have been associated with cancer progression. DSG3 is primarily involved in the formation of intercellular junctions, particularly cell-cell junctions, between epithelial, cardiomyocyte and other cell types. DSG3 is also involved in cell signaling pathways that control proliferation, differentiation and migration of keratinocytes. (PMID: 36375372) DSG3 is clinically associated with autoimmune blistering skin diseases.

References:

1. Viehweger F,et al. Pathol Res Pract. 2022;240:154200.                                            
2. Schmitt T,et al. Front Immunol. 2022;13:884241.                                                       
3. Schmitt T, et al. Front Immunol. 2023;14:1163066.