Recombinant Human EDDM3B protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg2443
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human EDDM3B protein Lys26-Asn147 (Accession# P56851) with a rabbit IgG Fc tag at the C-terminus. |
| GeneID | 64184 |
| Accession | P56851 |
| PredictedSize | 41.2 kDa |
| SDS-PAGE | 40-50 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
EDDM3B (Epididymal secretory protein E3-beta), also known as FAM12B, HE3B (Human epididymis-specific protein 3-beta), is highly enriched in the epididymis and is involved in the secretion of glycoproteins that interact with sperm membranes.
References:
1. Dacheux, Jean-Louis et al. Theriogenology vol. 63,2 (2005): 319-41. 2. Barrachina, F et al. Human reproduction (Oxford, England) vol. 37,4 (2022): 651-668.