Speedy™ Human Factor IX/PTC One-Step ELISA Kit
Cat no : SE50116
Synonyms
F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain
Validation Data Gallery
Product Information
| Product name | Speedy™ Human Factor IX/PTC One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 109.5-7000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2158 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 84%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3,564.2 | 104.1 | 2.9 |
| 2 | 8 | 819.6 | 14.8 | 1.8 |
| 3 | 8 | 425.1 | 7.7 | 1.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3,663.4 | 147.2 | 4.0 |
| 2 | 16 | 838.7 | 26.4 | 3.1 |
| 3 | 16 | 441.8 | 19.8 | 4.5 |
Background Information
Factor IX, also known as PTC, circulates as an inactive zymogen until the proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Upon activation by Factor XIa or Factor VIIa/tissue factor complex, Factor IXa forms a complex with Factor VIIIa, calcium ions, and phospholipids on the surface of activated platelets. This complex, known as the tenase complex, efficiently activates Factor X to Factor Xa, leading to thrombin generation and fibrin clot formation. Deficiencies or mutations in Factor IX result in Hemophilia B, an X-linked recessive bleeding disorder.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | F9, Factor IX, Christmas factor, coagulation factor IX, Coagulation factor IXa heavy chain |