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Recombinant Human Fas/CD95 protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 1-5 ng/mL

Cat no : Eg0877

Validation Data Gallery

  • Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

    Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.
Expression HEK293-derived Human Fas protein Gln26-Asn173 (Accession# P25445-1) with His tag at the C-terminus.
GeneID 355
Accession P25445-1
PredictedSize 17.4 kDa
SDS-PAGE 20-36 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Fas, also known as TNFRSF6, CD95, and APO-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis plays a role in the maintenance of cell homeostasis and in the deletion of useless or autoreactive T cells. Alterations in the CD95/CD95L pathway have been involved in several disease conditions, including autoimmune diseases, chronic inflammation and cancer.

References:

1.N Itoh. et al. (1991). Cell. 66(2):233-43.
2.M E Peter. et al. (2003). Cell Death Differ. 10(1):26-35.
3.M E Peter. et al. (2015). Cell Death Differ. 22(4):549-59.
4.Vesna Risso. et al. (2022). Cell Death Dis. 13(3):248.