Recombinant Human GGT1 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg4602

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Validation Data Gallery

• 

  Purity of Recombinant Human GGT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human GGT1 protein Pro27-Tyr569 (Accession# P19440-1) with a rabbit IgG Fc tag at the N-terminus.
• GeneID: 2678
• Accession: P19440-1
• PredictedSize: 85.8 kDa
• SDS-PAGE: 20-23 kDa and 70-90 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Glutamyltransferase 1 (GGT1) is abundantly expressed in various tissues including the pancreas. GGT1 is thought to be involved in the transport of amino acids across the plasma membrane through the gamma-glutamyl cycle. The main function of normal GGT1 is to induce glutathione molecules followed by providing protection against oxidative stress. It is known that oxidative stress plays a role in the pathogenesis of acute pancreatitis. Additionally, smokers with the GGT1 SNP rs5751901 show a higher risk for acute pancreatitis. Furthermore, GGT1 is involved in NF-κB pathway in a manner dependent on reactive oxygen species (ROS) regulation, although GGT1-mediated glutathione molecules suppressed oxidative stress.

References:

1. Heisterkamp N. et al. (2008). Hum Genet. 123(4):321-332.
2. Furukawa R. et al. (2024). Sci Rep. 14(1):12224.