Recombinant Human GPR56 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2478

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Validation Data Gallery

• 

  Purity of Recombinant Human GPR56 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human GPR56 protein Arg26-Asn171 (Accession# Q9Y653-1) with a rabbit IgG Fc tag at the C-terminus
• GeneID: 9289
• Accession: Q9Y653-1
• PredictedSize: 42.2 kDa
• SDS-PAGE: 45-55 kDa, reducing (R) condition
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

GPR56 (G-protein-coupled receptor 56, also named ADGRG1) is a member of the adhesion G-protein coupled receptor (aGPCR) family and one of the important players in the normal development of the brain. It plays a pivotal role in diverse neurobiological processes, including cortical formation, oligodendrocyte development, and myelination. Moreover, GPR56 has been reported to regulate VEGF secretion and angiogenesis in melanoma.

References:

1. Ganesh, Raksha A et al. Brain research vol. 1747 (2020): 147055.
2. Wu, Jinshan et al. Diabetes vol. 72,11 (2023): 1652-1663.