Recombinant human GPR56 protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
C-rFc
Activity
not tested
Cat no : Eg2478
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human GPR56 protein Arg26-Asn171 (Accession# Q9Y653-1) with a rabbit IgG Fc tag at the C-terminus |
| GeneID | 9289 |
| Accession | Q9Y653-1 |
| PredictedSize | 42.7 kDa |
| SDS-PAGE | 45-55 kDa, reducing (R) condition |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
GPR56 belongs to the adhesion family of G protein-coupled receptors (GPRs), is a receptor involved in cell adhesion and probably in cell-cell interactions, and mediates cell matrix adhesion in developing neurons and hematopoietic stem cells. GPR56 binding to the COL3A1 ligand inhibits neuronal migration and activates the RhoA pathway by coupling to GNA13 and possibly GNA12. GPR56 Plays a critical role in cancer progression by inhibiting VEGFA production thereby inhibiting angiogenesis through a signaling pathway mediated by PRKCA.
References:
1. Rong Luo, et al. (2012) PLoS One. 7(1):e29818. 2. Lei Xu, et al. (2006) Proc Natl Acad Sci U S A. 103(24):9023-8. 3. Liquan Yang, et al. (2011) Cancer Res. 71(16):5558-68.