Recombinant Human Granzyme B protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3360

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Validation Data Gallery

• 

  Purity of Recombinant Human Granzyme B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human Granzyme B protein Gly19-Tyr247 (Accession# P10144) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 3002
• Accession: P10144
• PredictedSize: 52.0 kDa
• SDS-PAGE: 55-65 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Granzyme B (GZMB) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease Granzyme B (GZMB) can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B also functions as processing protease of cytokines, including interleukin (IL)-18 and IL-1a, and extracellular matrix proteins, including fibronectin and various proteoglycans. Plasma Granzyme B concentrations were significantly higher in patients with Atopic dermatitis (AD) and psoriasis than in healthy controls, which indicated that Granzyme B may play an important role in the induction of intractable itch and dermatitis in AD.

References:

1.Hiebert, Paul R, and David J Granville. (2012) Trends Mol Med. 18(12):732-741. 
2.Kamata, Yayoi et al. (2016) J Dermatol Sci.84(3):266-271.