Human HSP60 ELISA Kit

Catalog number: KE00032

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$599.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human HSP60 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma

Assay type:
Sandwich

Sensitivity:
7 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 99% 86%-119%

Product overview:
KE00032 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSPD1 ELISA kit is to be used to detect and quantify protein levels of endogenous HSPD1. The assay recognizes human HSPD1. A polyclonal antibody specific for HSPD1 has been pre-coated onto the microwells. The HSPD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HSPD1 is added to detect the captured HSPD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
60 kDa chaperonin, Chaperonin 60, CPN60, GROEL, Heat shock protein 60, HLD4, HSP 60, HSP60, HSP65, HSPD1, HuCHA60, P60 lymphocyte protein, SPG13
Background

HSPD1(also known as HSP60) belongs to the chaperonin family and acts as a chaperone essential for protein folding. HSP60 is a widely expressed protein and has been connected with many aspects of cell functions including gene expression regulation, DNA replication, signal transduction, differentiation, apoptosis, and cellular senescence or immortalisation. Recently it has been reported that HSP60 was significantly up-regulated in squamous cell lung cancer.


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